GSM5717968: KO rep2; Mus musculus; RNA-Seq - SRA

SRX13317848: GSM5717968: KO rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 24.5M spots, 1.2G bases, 409.9Mb downloads

External Id: GSM5717968_r1

Submitted by: Pathology and Immunology, Washington University

Study: Expression profiling of miR-142 deficient Treg cells

PRJNA786239 • SRP349261 • All experiments • All runs

show Abstracthide Abstract

miR-142 gene is specifically and abundantly expressed in hematopoietic lineages. Mice with specific deletion of this miRNA gene in Treg develop fatal autoimmunity. We used RNA-seq to detect whole transcriptome changes in miR-142 null Treg cells. Overall design: Total RNA from sorted CD4+YFP+ Treg cells (Foxp3Cre-YFP,n=3 and Foxp3Cre-YFPmiR-142fl/fl, n=3) was extracted and subjected to RNA-seq. Samples in Foxp3Cre and Foxp3CremiR-142fl/fl groups are biological replicates and were isolated in age and gender matched mice.

Sample: KO rep2

SAMN23637688 • SRS11226032 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM5717968

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was isolated using miRNeasy kit (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols

Runs: 1 run, 24.5M spots, 1.2G bases, 409.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR17133389	24,476,544	1.2G	409.9Mb	2021-12-07

ID:: 18224859

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